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pH stable HILIC
Posted: Tue Jan 24, 2012 4:10 pm
by DJ
Are there non-silica HILIC columns? Could a methacrylate polymeric support work in HILIC mode?
What about a PSDVB base material made with penta hydroxymethyl styrene? Could this work as a stationary phase for HILIC separation?
Re: pH stable HILIC
Posted: Tue Jan 24, 2012 9:44 pm
by Andy Alpert
DJ:
Almost anything can be made to work in the HILIC mode except for a totally encapsulated C-18 material, provided you're willing to use a level of organic solvent that's high enough. Whether it's a good idea to use such is another matter. The less hydrophilic a material is - per these polymeric materials - the more organic solvent you need to get a given degree of retention of an analyte. What's so interesting about these materials?
Re: pH stable HILIC
Posted: Wed Jan 25, 2012 3:24 pm
by carls
Sequant has a polymeric HILIC column, the ZIC p-HILIC. Usually polymeric columns are used when silica columns are too active, such as with phosphates, phosphonates, etc. Otherwise, silica columns offer a wider variety of chemistires and typically better performance.