Chromatography Forum

Hallo everybody,
I have a question:
do you know the extinction coefficient of malondyaldehyde or iUPAC name: propanedial at 532nm?
or do you know how can I determine it?
I know the Lambert-Beer Law, but it doesn't determine with inverse formula!!!
Thank you so much for your attenction.
I hope hearing you soon.

Hi

Sure it does, though it is easy to use the wrong units.

extinction coefficient= A/b*c where:
A=absorbance
b=cell pathway in cm
c=concentration in mol/L

Also note that the extinction coefficient is only valid for the given wavelength and given solvent, if changed the extinction coefficient will likely change.