What is baseline resolution?
Posted: Sun Jan 15, 2012 7:04 am
In chromatography, what does "baseline" resolution mean?
In principle, it's just what it sounds like: the amount of resolution between adjacent peaks at which the signals will drop to the baseline.
In practice, many textbooks will state this value as Rs = 1.5 . This is a bit misleading; it is the resolution at which two equal-sized Gaussian peaks will have less than 1% overlap. Decades ago, this used to be called "99% baseline resolution" (for obvious reasons); over the intervening years, the "99%" part got dropped off and it's just called "baseline resolution".
And, of course, you know that resolution (Rs) is the ratio of center-to-center separation to average baseline width for two adjacent peaks.