aminoacid separation 100% aueous phase

[Ttati]

I need to separate amino acids but only with non organic eluents, so without methanol or acetonitryle, so 100% aqueous phase. I can use only buffer. i found some separation like that with Primesep A column, i wonder what other columns can i use?

[tom jupille]

Cation exchange. Check the BioRad catalog for "Aminex" resins.

[Vlad Orlovsky]

What is wrong with Primesep A. James McCullagh and his team published several articles on separation of amino acids in zero organic mode using Primesep A mixed-mode columns. They did intensive prep separations too.

1. "Analysis of amino acid 13C abundance from human and faunal bone collagen using liquid chromatography/isotope ratio mass spectrometer", James S. O. McCullagh, Dieter Juchelka, Robert E. M. Hedges, Rapid Communications in Mass Spectrometry, Volume 20, Issue 18 , Pages 2761 - 2768

2."Determination of underivatized amino acid 13C by liquid chromatography/isotope ratio mass spectrometry for nutritional studies: the effect of dietary non-essential amino acid profile on the isotopic signature of individual amino acids in fish", James McCullagh, Julia Gaye-Siessegger, Ulfert Focken, Rapid Communications in Mass Spectrometry, Volume 22 Issue 12, Pages 1817 - 1822

3. "Mixed-mode chromatography/isotope ratio mass spectrometry", James S. O. McCullagh, Rapid Communications in Mass Spectrometry
Volume 24, Issue 5, pages 483–494, 15 March 2010

4. "Liquid chromatography/isotope ratio mass spectrometry measurement of δ13C of amino acids in plant proteins", Anthony H. Lynch, James S. O. McCullagh, Robert E. M. Hedges, Rapid Communications in Mass Spectrometry, October 2011, Vol. 25, No. 20, p 2981-2988

5. "Review: Current applications and challenges for liquid chromatography coupled to isotope ratio mass spectrometry (LC/IRMS)", Jean-Philippe Godin, James S. O. McCullagh, Rapid Communications in Mass Spectrometry, October 2011, Vol. 25, No. 20, p 3019-3028

Contact me if you have any questions.