Cleaning HPLC, detergent

[rjr2259]

Hello,
I have injected 0.5M Sodium Laurly sulfate on my HPLC as a mobile phase. How can I clean out the system?

[BostonFSE]

isnt that a foaming agent for soaps and shampoo? you made a mobile phase out of that? why?
hot water flush would be my first attempt to remove it.

[rjr2259]

Thank you for your suggestion,
I have flushed over night with water, will any other solvents aid in SLS removal?

Thank you

[Consumer Products Guy]

isnt that a foaming agent for soaps and shampoo? you made a mobile phase out of that? why?

Maybe poster was using as an ion-pairing reagent.

Anyway, I'd do water rinse followed by methanol rinse. The anionic surfactant is soluble in those.

[DR]

isnt that a foaming agent for soaps and shampoo? you made a mobile phase out of that? why?
hot water flush would be my first attempt to remove it.

It also shows up once in a while as a dissolution buffer additive, but never at anything approaching 0.5M - this concentration seems quite high for use in LC... I suspect lots of patience and varying MeOH/Water rinse will (eventually) get it out.

[rjr2259]

I suspected as much, It has been flowing water for about 24 hours straight now. I plan to run it over the weekend as well, probably 5ml/min.

[JMB]

Working from memory of 12yrs ago, I would recommend a 50/50 mix of 50 mMolar Ammonium acetate aqueous and MeCN.
Set col temp at 50 C so that you can increase flow rate to about 2 mL/min.
I don’t think that you will be able to pump water at 5 mL/min through a true HPLC column.
Are you using SEC ?

Regards,
JMB

[rjr2259]

Currently I do not have a column on the system, I am just pumping solvent through it. JMB, your suggestion of pumping 50/50 ammonium acetate and ACN, was that used specifically for detergent removal?

[JMB]

Yes.
It may have been aq. Am Acetate and MeOH
As I say, from memory.
JMB