How to determine Limit of Quantitaion (LOQ)

[isaac.lee]

I am developing a GC method and I am trying to determine LOQ/LOD. What will be the proper steps to determine LOQ/LOD?

[DR]

Step one - search these very forums for just your topic. Links to tutorials etc. have been posted before (a few times).

[wiclef]

EPA proposed that the minimum detection limit is equal to the standard deviation limit multiply the student t value from statistical tables for 99% confidence level and n-1 degrees of freedom.
The minimum level of quantification ML is equal to 10 multiply to the standard deviation but normally we calculate the ML from the MDL.for example a sample size of 7, the MDL = 3.143 * S so ML = 10 MDL/3.143=
3.18 * MDL.

I think that I give you the answer so try to do this.In addition, the procedure is not harmonized so you judge yourself.

Wiclef Kagisha

[Hollow]

Step one - search these very forums for just your topic. Links to tutorials etc. have been posted before (a few times).