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- Posts: 42
- Joined: Wed Sep 14, 2011 1:47 pm
Hi everyone, today I had a strange problem with early peaks. I used my Mobile phase as a dissolvent and a blank sample.
In my blank sample, there is always one peak at 1.5' with the area is 10000 and that peak also present in my test sample with the same Retention time but different from area. Here is my ordered injection :
Blank (x1) > Standard (x6) > Sample (x2) > Standard (x1)
When I inject the Blank sample, I get a peak with area is about 10000. If I inject my Test Sample right after my Blank, the area of peak at 1.5' in Test Sample is equal with the one in Blank sample both retention time and area.
But when I inject follow my ordered injection, the area of peak at 1.5' in Test Sample or Blank Sample is increased a lot ( about 20000 in area)..
I think that peak increases with the number of my injections which means the more I inject, the bigger area value I got from the peak at 1.5' ( both in Blank Sample and test sample). Could you please help me find out the reason in order to avoid it next time. Thanks in advanced
In my blank sample, there is always one peak at 1.5' with the area is 10000 and that peak also present in my test sample with the same Retention time but different from area. Here is my ordered injection :
Blank (x1) > Standard (x6) > Sample (x2) > Standard (x1)
When I inject the Blank sample, I get a peak with area is about 10000. If I inject my Test Sample right after my Blank, the area of peak at 1.5' in Test Sample is equal with the one in Blank sample both retention time and area.
But when I inject follow my ordered injection, the area of peak at 1.5' in Test Sample or Blank Sample is increased a lot ( about 20000 in area)..
I think that peak increases with the number of my injections which means the more I inject, the bigger area value I got from the peak at 1.5' ( both in Blank Sample and test sample). Could you please help me find out the reason in order to avoid it next time. Thanks in advanced
