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question

Posted: Tue Dec 13, 2011 12:52 am
by mona
how eluent composition affects capacity factor and resolution?

how can i answer this question? :shock:

thanks alot

Re: question

Posted: Wed Dec 14, 2011 4:00 am
by chromatographer1
I assume you are discussing TLC or LC.

Changing the eluent composition affects the retention time and the resolution of analytes.

Specifics depend upon the nature of the chromatography you are using. Normal phase, reversed phase, ion exchange, etc.

These issues are discussed in any elementary textbook on chromatography.

best wishes,

Rod