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Cholorofrom and UV absorption at 234 nm

http://www.chromforum.org/viewtopic.php?t=18483

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Cholorofrom and UV absorption at 234 nm

Posted: Mon Dec 12, 2011 8:37 am
by jusuom
I need to use UV detector to record arborption at 234 nm. One of my solvents contains some chloroform. Supposedly chloroform absorbs heavily at this wavelength. Any suggestions what solvent to try instead?

What kind of solvent system (with non-chorinated solevnets) would you recommend for resolving lipid classes by normal-phase HPLC?

Re: Cholorofrom and UV absorption at 234 nm

Posted: Mon Dec 12, 2011 4:28 pm
by tom jupille
One of the problems with normal-phase in general is that many (if not most) suitable solvents have UV-cutoff issues. My first suggestion would have been methylene chloride. Other than that, you can pick something of similar polarity from the list:
http://murov.info/orgsolvents.htm#TABLE%202
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