Chromatography Forum

Hi everybody,
I´m chemist at the University of Antioquia, Medellín Colombia, Faculty of Animal Science, I get an HPLC agilent Tech with Fluorescence Detector, I have two questions for you,the first question is:how long is the lifetime of the lamp , and second one is:what occurs when the luminiscence is too high when I inject a sample , these changed to very low luminiscence values in one moment to another?.
I preciate your help.
Until then.
Silvio

I don't know, but I would suggest looking here...http://www.chem.agilent.com/scripts/lit ... nfotype=99

Again me,
In the manual you say don´t appears the answer I looking for, I have been search this information in other sources but I didn´t found anything about the topic I mentioned.
I hope any help me if anyone get some experience with that question.
Thanks a lot,
Silvio.

Never having worked with an Agilent only a general answer is possible.
Lamp manufacturers usually state a lamp life, which is usually far surpassed (it´s like gambling). Now I don´t know about this "luminescence", but it would seem logical that it means that the emission is too strong to be measured. If this appears during the peak just inject less, if it appears some other time you could be close to the exciting wave length or its overtone, or there could be fluorescing "dirt" coming through.

Ups:
Being too close to the excitation or overtone would be alleviated only if you ran a spectrum, not during a chromatogram which is run at a permanent emission wavelength.

Thanks a lot for your help,
It was very helpful for me, but I will searching on this information, If I know something, I¨ll send you what I found in this topic, I think that lamp is over when the signal have no changes in the calibration and of course the response in the spectrum doesn´t emit any peak.
Again, thank you for show me a light in this search
Silvio