Chromatography Forum

Hi guys,

wonder if any of you can help us, we have recently connected our agilent to our chromeleon software pc (it was running on agilent chemstation) and it works fine on UV. We've tried to connect the CAD detector now through a direct USB connection as we did for the chemstation set up. It works and we have data. We have now however tried to set off a run and it injects and data recording works however after the first injection is "times out" and wont go on to the next one. The program file and error log are as below, any suggestions as to what this could be?! We've all but given up trying to contact Dionex they never call back despite their promises so we're very stuck!

Many thanks,
lynz x


our program file:
ColumnComp.TempCtrl = On
Mode = Combined
LeftTemperature.Nominal = 40.00 [°C]
LeftTemperatureDelta = 0.80 [°C]
LeftTemperature.LowerLimit = -5.00 [°C]
LeftTemperature.UpperLimit = 80.00 [°C]
Pressure.LowerLimit = 0.0 [bar]
Pressure.UpperLimit = 400.0 [bar]
MaximumFlowRamp = 100.000 [ml/min²]
%A.Equate = "%A"
%B.Equate = "%B"
%C.Equate = "%C"
%D.Equate = "%D"
PrimaryChannel = Automatic
Compressibility = Automatic
Stroke = Automatic
Sampler.TempCtrl = Off
DispSpeed = 200 [µl/min]
DrawSpeed = 200 [µl/min]
SampleHeight = 0.0 [mm]
WashVial = None
Data_Collection_Rate = 10 [Hz]
CAD_1.FilterConstant = None
Flow = 1.000 [ml/min]
%B = 0.0 [%]
%C = 0.0 [%]
%D = 100.0 [%]

0.000 CAD.Autozero
Wait UV.Ready and CAD.Ready and ColumnComp.Ready and Pump.Ready and Sampler.Ready
Inject
CAD_1.AcqOn

1.000 CAD_1.AcqOff

End

The agilent log messages:

[Message] 12:24:49 Batch started.
[Message] 12:24:49 Start of sequence "SEQ::\ER41-63_local\ScaledSol_IC_UV\011211 863 SAX Agilent CAD".
[Message] 12:24:49 Start of sample 1 "blank (RO)", using program "Agilent CAD UPpH7 line D check".
[] 12:24:49 0.000 ColumnComp.TempCtrl = On
[] 12:24:49 0.000 Mode = Combined
[Command] 12:24:49 0.000 LeftTemperature.Nominal = 40.00
[Command] 12:24:49 0.000 LeftTemperatureDelta = 0.80
[Command] 12:24:49 0.000 LeftTemperature.LowerLimit = -5.00
[Command] 12:24:49 0.000 LeftTemperature.UpperLimit = 80.00
[Command] 12:24:49 0.000 Pressure.LowerLimit = 0.0
[Command] 12:24:49 0.000 Pressure.UpperLimit = 400.0
[] 12:24:49 0.000 MaximumFlowRamp = 100.000
[Command] 12:24:49 0.000 %A.Equate = "%A"
[Command] 12:24:49 0.000 %B.Equate = "%B"
[Command] 12:24:49 0.000 %C.Equate = "%C"
[Command] 12:24:49 0.000 %D.Equate = "%D"
[] 12:24:49 0.000 PrimaryChannel = Automatic
[] 12:24:49 0.000 Compressibility = Automatic
[] 12:24:49 0.000 Stroke = Automatic
[] 12:24:49 0.000 Sampler.TempCtrl = Off
[] 12:24:49 0.000 DispSpeed = 200
[] 12:24:49 0.000 DrawSpeed = 200
[] 12:24:49 0.000 SampleHeight = 0.0
[Protocol] 12:24:49 0.000 WashVial = None
[] 12:24:49 0.000 Nebulizer_TempCtrl = Off
[Protocol] 12:24:49 0.000 Data_Collection_Rate = 10
[Command] 12:24:49 0.000 CAD_1.FilterConstant = None
[Command] 12:24:49 0.000 Flow = 1.000
[Command] 12:24:49 0.000 %B = 0.0
[Command] 12:24:49 0.000 %C = 0.0
[Command] 12:24:49 0.000 %D = 100.0
[Command] 12:24:49 0.000 CAD.Autozero
[Command] 12:24:49 0.000 Wait UV.Ready and CAD.Ready and ColumnComp.Ready and Pump.Ready and Sampler.Ready
[Message] 12:24:54 0.000 Wait finished
[Command] 12:24:54 0.000 Inject
[Message] 12:24:54 0.000 {Sampler} Injecting from vial position 1.
[Message] 12:24:54 0.000 {Sampler} Injection Volume is 100.0 µl.
[Message] 12:24:54 0.000 {Sampler} Waiting for inject response on HP1100InjectState.
[Error] 12:24:56 0.000 {Sampler} Communication error: LICOP command not acknowledged.
(option not installed )
[Message] 12:26:36 0.000 {Sampler} Got inject response.
[Command] 12:26:36 0.000 CAD_Relay_1.State = On
[Command] 12:26:36 0.000 CAD_1.AcqOn
[] 12:26:36 0.000 {CAD_1} Log Step: 0.10 [s]
[Command] 12:27:06 0.500 CAD_Relay_1.State = Off
[Command] 12:27:06 0.500 CAD_1.AcqOff
[Message] 12:27:12 0.590 {CAD} Terminating program PGM::\ER41-63_local\ScaledSol_IC_UV\011211 863 SAX Agilent CAD.SEQ\Agilent CAD UPpH7 line D check. Waiting for instrument to finish processing of current sample ...
[Message] 12:27:12 0.591 {CAD} OK to terminate program PGM::\ER41-63_local\ScaledSol_IC_UV\011211 863 SAX Agilent CAD.SEQ\Agilent CAD UPpH7 line D check.
[Abort] 12:27:22 0.757 {CAD} Timeout while waiting for the instrument to finish, the sample will be aborted.
[Abort] 12:27:22 0.757 {CAD} Timeout while waiting for the instrument to finish, the sample will be aborted.
[Abort] 12:27:22 0.758 {CAD} Timeout while waiting for the instrument to finish, the sample will be aborted.
[Abort] 12:27:22 0.758 {CAD} Timeout while waiting for the instrument to finish, the sample will be aborted.
[Message] 12:27:22 End of sample "blank (RO)".
[Error] 12:27:22 Batch stopped.
[Message] 12:27:23 {Sampler} Device notification ( Aborting active )
[Message] 12:27:29 {Sampler} Device notification ( Aborting active )

I'd call Agilent about this before calling Thermo/Dionex...

Hi all,

we've managed to get a call back from Dionex today who have passed us to their CAD expert and is calling us back this aftenoon to try to sort out our issue, looks like its a missing cable on top of the one we have.

We have also just been called by Norman Ramsey from Thermo Fisher who chased us down from this post on this forum and has suggested that it is indeed a cable we're missing and offered to forward us a link and instructions for fitting the cable! Very helpful service from him so very grateful wish we'd thought of contacting him first off!

x