Low pH modifiers: TFA vs. Perchloric Acid - Baseline Quality
Posted: Thu Dec 01, 2011 4:23 pm
More and more we seem to have issues baseline quality (humps, bumps, steps etc.) with generic gradient conditions with 0.05%/0.1% TFA containing mobile phases @ low wavelengths e.g. 220 nm.
I was wondering if we would see less issues if we'd move to using something like 0.05% perchloric acid instead. Has anyone tried something like this and if so did it improve or remove the various baseline artifacts common with TFA containing eluents?
I was wondering if we would see less issues if we'd move to using something like 0.05% perchloric acid instead. Has anyone tried something like this and if so did it improve or remove the various baseline artifacts common with TFA containing eluents?