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- Posts: 1
- Joined: Wed Nov 30, 2011 5:45 am
Hello everyone
Today I encountered a problem while using the LC/MS/MS which I have not previously encountered. The system is an Agilent1100 coupled with a QTrap
Prior to every experiment, I do system validation with reserpine. However on doing that today, I did not observe any peak.Instead I see intermittent tiny peaks (of about 10-30cps) unlike my previous peak (of over 1000cps)Also, previously, I had just one peak at abt 25sec.However, today I saw about 5 little peaks at different times. I have done all the troubleshooting I can think of but no progress yet
Please has anyone encountered this before?How was it resolved????...or any recommendations/suggestions
I cant do further experiments because of this as my sample also is not giving my desired peak
Thank you
Today I encountered a problem while using the LC/MS/MS which I have not previously encountered. The system is an Agilent1100 coupled with a QTrap
Prior to every experiment, I do system validation with reserpine. However on doing that today, I did not observe any peak.Instead I see intermittent tiny peaks (of about 10-30cps) unlike my previous peak (of over 1000cps)Also, previously, I had just one peak at abt 25sec.However, today I saw about 5 little peaks at different times. I have done all the troubleshooting I can think of but no progress yet
Please has anyone encountered this before?How was it resolved????...or any recommendations/suggestions
I cant do further experiments because of this as my sample also is not giving my desired peak
Thank you
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