Reduce the memory effect
Posted: Tue Nov 22, 2011 10:42 am
Hi everybody,
Two words about me : my name is Jean, validation operator, working in a Belgian Biotech compagny which especially produces oligonucleotides for research use or IVD.
For some kinds of oligos purified on RP-HPLC, I have a high "memory effect" because these are modified with strong hydrophobic dyes.
I'm working with a gradient of 2 solutions (A :TEAA in water/ACN and B: ACN). My gradient goes to 100% of B in 10 min and last for 1 min. then go back to 100% A (equilibration for the next sample).
Is it possible for to ask you giving me some clues to reduce this memory effect observed with these oligos.
Thanking you in advance!
Jean
Two words about me : my name is Jean, validation operator, working in a Belgian Biotech compagny which especially produces oligonucleotides for research use or IVD.
For some kinds of oligos purified on RP-HPLC, I have a high "memory effect" because these are modified with strong hydrophobic dyes.
I'm working with a gradient of 2 solutions (A :TEAA in water/ACN and B: ACN). My gradient goes to 100% of B in 10 min and last for 1 min. then go back to 100% A (equilibration for the next sample).
Is it possible for to ask you giving me some clues to reduce this memory effect observed with these oligos.
Thanking you in advance!
Jean
