Waters HPLC system

[s10pd021]

Hi

I am using a Waters 2489 UV/Vis detector and been having a lot of problems with it - mainly inconsistencies in separation if any at all. Also, their Autosampler 2707 doesn't seem to allow me to make more than 50uL injections although it has a 100uL sample loop.

Has anyone been having similar problems with Waters?

[paulw]

Check the settings in your method to see if you are set to use a full loop or partial loop. Oftentimes if the method in the software is set to partial loop on a 100uL loop, then it will allow 50uL as the max injection, but if you change it to full loop you can do the 100uL injection.

[tom jupille]

Also, the detector has nothing to do with inconsistencies in the separation. It merely sees what's coming out of the column.

[unmgvar]

the 2707 is an OEM from spark holland
http://www.sparkholland.com/products/au ... ers/aliast

this sampler is a partial or full loop sampler
so you can inject up to 50ul or a 100ul
you can probably inject also in between but due to the design of the sampler the RSD will not be great