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Separation of natural glycosides.

http://www.chromforum.org/viewtopic.php?t=18290

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Separation of natural glycosides.

Posted: Sat Nov 19, 2011 10:42 pm
by chemwest
Hello
I am trying to purify glycosides from natural source. Some of the glycosides are very closely related by mass and its very close on HPLC. Mobile phase used was Acetonitrile - water and 0.1% TFA and Zorbax ODS 5micron column. Some compounds separate while others (larger ones) tail.
Suggestions and ideas welcome for better separation.
Thanks

Re: Separation of natural glycosides.

Posted: Sun Nov 20, 2011 11:10 am
by unmgvar
what is the biggest Mw of the compounds?

Re: Separation of natural glycosides.

Posted: Mon Nov 21, 2011 10:28 pm
by chemwest
According to LC-MS the m/z are 740, 755 and 817.
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