Hello, I have very little DAD experience, so my apologies if my question is trivial.

I have heard conflicting "practices" that if you collect the spectrum from 190 to 400nm, the peak of interest should be pure (ie a threshold of 990-995) across the entire spectrum.

I have also heard that another "practice" is that purity only needs ot be demonstrated (ie threshold of 990-995) at least +/- 10nm from the acquisition wavelength.

Would love to hear some thoughts.

Thanks in advance.