Chromatography Forum

Hello there guys

Had a question

Does anyone have any recommendation about an appropriate column for the analysis of Naphazoline Hydrochloride?
USP recommends L1 which I think is the RP C18 (25 cm x 4.0 mm)- Are there any other ones?
I would really appreciate if I can get any feedback-

Thank you in advance

PFP propyl columns can also be used for this.
ABSciex includes this analyte in their screening method for drugs of abuse:

http://www.absciex.com/Documents/Produc ... 210-01.pdf

Try searching in phenomenex page. There are some drug applications there. Perhaps you can found Napahazoline.

You can use a mixed-mode column to determine naphazoline. Here is some information on Acclaim Trinity P1 column: http://www.dionex.com/en-us/webdocs/707 ... 239-02.pdf. Figures 7 and 8 are more related to your application.

Acclaim Trinity P1 provides reversed-phase/anion-exchange/cation-exchange retention at the same time, and is speciaully designed to determine API and counterion.

Here are some references on this column in case your are interested:
1. K. Zhang, L. Dai, N. P. Chetwyn, Simultaneous determination of positive and negative pharmaceutical counterions using mixed-mode chromatography coupled with charged aerosol detector, J. Chromatogr. A 1217 (2010) 5776 – 5784.
2. X. Liu, C. Pohl, HILIC behavior of a reversed-phase/cation-exchange/anion-exchange trimode column, J. Sep. Sci. 33 (2010) 779 –786.
3. B. N. Tran, R. Okoniewski, R. Storm, R. Jansing, K. M. Aldous, Use of Methanol for the Efficient Extraction and Analysis of Melamine and Cyanuric Acid Residues in Dairy Products and Pet Foods, J. Agric. Food Chem. 58 (2010) 101 – 107.
4. X. Liu, C. Pohl, A. Woodruff, J. Chen, Chromatographic Evaluation of Reversed-Phase/Anion-Exchange/Cation-Exchange Trimodal Stationary Phases Prepared By Electrostatically Driven Self-Assembly Process, J. Chromatogr. A. 1218 (2011) 3407.

Are you bound to the USP monograph (i.e. are you working in a regulated environment) or are you free to change the method?
If you must stick to the monograph you MUST use a L1 aka C18 column, mixed mode column will not be acceptable. USP recommends LiChroSpher RP18e to be used. This is quite an old column, a modern one might yield nicer chromatograms. You're free to use any column you want, as long as it is C18 25x4.0mm as written in the monograph (you may change the dimensions according to USP's general chromatography chapter though).
I'd try the recommended one first and only if chromatography is unacceptable (which might be the case although it's the recommended one ) try different columns.

If you're free to change the method a good starting point is to roam the column manufacturers databases, as already mentioned.