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How to make it hydrophobic ?
Posted: Mon Nov 14, 2011 10:20 am
by Newchromatographer
Hi folks ,
How to make this compound hydrophobic to transfer it from aqueous phase to organic phase in seperatory funnels ? a mide group ( pKa= 10.99 ).
not sure if low ph going to help ?
Re: How to make it hydrophobic ?
Posted: Mon Nov 14, 2011 1:30 pm
by Kristof
In the source you provided, just below pKa, you can find this bit:
Partition coefficient, pKow
3.68
ie. it is already
quite hydrophobic IMHVO...
Re: How to make it hydrophobic ?
Posted: Mon Nov 14, 2011 10:32 pm
by Newchromatographer
I used different immiscible solvents ( hexan , , ethyle acetate Toluene etc ) in distilled water but the more i get is 60% and i need 100% or near to it , do u think if i use ph 3 this gonna help ?
i would be gratefull if you illustrate it more ,,
Re: How to make it hydrophobic ?
Posted: Tue Nov 15, 2011 7:59 am
by Kristof
That's 60% for single, double, triple extraction?
Re: How to make it hydrophobic ?
Posted: Tue Nov 15, 2011 10:55 pm
by Newchromatographer
triple extraction and extrat 10 ml solution by ( 10 ml solvent *3 )
Re: How to make it hydrophobic ?
Posted: Tue Nov 15, 2011 11:24 pm
by HPLCaddict
pH3 won't help you. I'd try saturating the aqueous phase with e.g. sodium chloride.
Re: How to make it hydrophobic ?
Posted: Wed Nov 16, 2011 11:47 am
by Johnny Rod
HPLC addict is right, saturate the aqueous phase with salt. It's a carbamate so isn't very basic anyway. Once you have it extracted into organic solvent then you may need to add anhydrous sodium sulfate and filter to remove water and inorganics, unless your further analysis/treatment can tolerate these.