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flow marker GPC with HFIP

http://www.chromforum.org/viewtopic.php?t=18149

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flow marker GPC with HFIP

Posted: Fri Nov 04, 2011 2:02 pm
by pieter
Hello everybody,

I'm developing a GPC method with hexafluoroisopropanol.
I was wondering what kind of flow marker is compatibel with this and what kind of concentrations are used.
The sample concentrations are 1 mg/ml (0.1 % w/v)
I also will contact the supplier of the collumns but any suggestion is more then welcome.

Kind regards,

Pieter.

Re: flow marker GPC with HFIP

Posted: Fri Nov 04, 2011 6:13 pm
by sepscientologist
I would think any sort of solvent molecule that shows up on your detector. Depends on your detector I suppose.
How about acetone?

Re: flow marker GPC with HFIP

Posted: Sun Nov 06, 2011 5:25 pm
by JokerB
HFIP is in-soluble in ACN, but works fine with MeOH. I dont know if these solvents are suitable for your application.
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