Seal Wash

[StephenO]

When using the Waters 2695 Autosampler does the Seal Wash actually enter into the Mobile Phase during a HPLC run (can seal wash affect the chromatogram) and how often does the system carry out a seal wash?

Thanks in advance

[aceto_81]

On a normal functional 2695, the seal wash is separated from the mobile phase system.
And as the mobile phase is at a much higher pressure than the seal wash, it's more the other way: the mobile phase leaks into the seal wash waste.

I think the default cycle time for the seal wash is 1 minute (you can set this manually)

Ace

[StephenO]

Thanks Ace

[Bull76]

If the seals are defunct then the seal wash can enter the mobile phase. Are you having an interaction based on the seal wash and your mobile phase combustion?