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Pore Size: GE HiTrap Ion Exchange
Posted: Sun Oct 23, 2011 8:31 am
by yolevins
I neglected to filter my sample through a 0.45 micron filter, and I'm worried my sample might be stuck inside my GE HiTrap Q 1 ml anion exchange column. (I'm working with Vitamin D, which forms aggregates larger than 0.45 microns...)
Is there any way for me to clean my column, or run it backwards?
Would particles larger than 0.45 microns actually get stuck in the column, or flow through?
Re: Pore Size: GE HiTrap Ion Exchange
Posted: Sun Oct 23, 2011 5:11 pm
by unmgvar
if i remember well we are talking of a something like 30u size resin in the column?
the rule of thumb is to filter 1/10 of the particle size because this is the expected size of the channels between the particles and not taking into account the frit type you have at the head of the column
i really do not remmeber the size of the frit.
Re: Pore Size: GE HiTrap Ion Exchange
Posted: Mon Oct 24, 2011 12:08 pm
by yolevins
The nominal particle size is 34 um. 1/10 of that is 3.4 um, or 3400 nm - in my case, that gives me plenty of leeway.
Why does the manual tell me to filter closer to 1/100 of the size if 1/10 is the rule of thumb?
Thanks!
Re: Pore Size: GE HiTrap Ion Exchange
Posted: Mon Oct 24, 2011 8:20 pm
by tom jupille
Why does the manual tell me to filter closer to 1/100 of the size if 1/10 is the rule of thumb?
They may be using a very small porosity frit. My suggestion would be "when in doubt, follow the manual".