unretained analyte for a 1000A SEC column

Discussions about gel permeation chromatography / gel filtration chromatography / size exclusion chromatography

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unretained analyte for a 1000A SEC column

: ginebra

: Posts: 12; Joined: Mon May 19, 2008 1:46 pm

by ginebra » Thu May 26, 2011 8:26 am

Hi,

Can anybody help me with finding an analyte to determine the exclusion volume for a 1000A pore size SEC column. The column can separate analytes up to 8MDa. Even the manufacturer cannot provide me with either information about exclusion volume nor analytes to use.
what could I use?

Cheers,
Chris

Re: unretained analyte for a 1000A SEC column

: unmgvar

: Posts: 835; Joined: Thu Apr 14, 2005 7:00 am

by unmgvar » Thu May 26, 2011 10:04 am

maybe polyanalityk can help you

http://www.polyanalytik.com/polymer-standard-kits.php

Re: unretained analyte for a 1000A SEC column

: ginebra

: Posts: 12; Joined: Mon May 19, 2008 1:46 pm

by ginebra » Tue May 31, 2011 2:06 pm

Their reply takes ages but thanks a lot for the link!!!

Cheers,
Chris

Re: unretained analyte for a 1000A SEC column

: Vlad Orlovsky

: Posts: 775; Joined: Mon Dec 01, 2008 9:59 pm

by Vlad Orlovsky » Tue May 31, 2011 2:32 pm

Their reply takes ages but thanks a lot for the link!!!

Cheers,
Chris

I guess people were in a Holiday mood, and thought about BBQ and beer

Vlad Orlovsky
HELIX Chromatography
My opinions might be bias, but I have about 1000 examples to support them. Check our website for new science and applications
www.helixchrom.com

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