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Hamilton prp columns
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
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Does anyone know the difference between hamilton prp x100 and prp-1? Because I need a L21 column for Alendronate USP assay and I have this two in my laboratory? Which one is better?
Q. F. Ignacio Viera
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- tom jupille
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The information is available on the Hamilton web site:
prp x100: http://www.hamiltoncompany.com/products ... ies/c/369/
prp1: http://www.hamiltoncompany.com/products ... ies/c/274/
The short answer is that PRP X100 is an anion exchange column; PRP1 is a reversed-phase column (entirely different animals!)
prp x100: http://www.hamiltoncompany.com/products ... ies/c/369/
prp1: http://www.hamiltoncompany.com/products ... ies/c/274/
The short answer is that PRP X100 is an anion exchange column; PRP1 is a reversed-phase column (entirely different animals!)
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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- Posts: 85
- Joined: Wed Oct 19, 2011 9:46 am
Thanks for your answer Tom. But, PRP X100 can still be used in reversed phase, isn´t it?The information is available on the Hamilton web site:
prp x100: http://www.hamiltoncompany.com/products ... ies/c/369/
prp1: http://www.hamiltoncompany.com/products ... ies/c/274/
The short answer is that PRP X100 is an anion exchange column; PRP1 is a reversed-phase column (entirely different animals!)
Q. F. Ignacio Viera
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- tom jupille
- Site Admin
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- Posts: 4978
- Joined: Wed Aug 11, 2004 4:55 pm
L21 is polystyrene (OK, PS-DVB). The PRP1 is clearly what is indicated for that classification. Hamilton claims the PRP X100 as also being L21, but I would argue that is inappropriate (the intent of L21 is a neutral resin).
Could the X100 be used is a reversed-phase column? Maybe, but only for *neutral* molecules. For ionized species I would expect ion-exchange or ion-exclusion behavior to predominate.
Could the X100 be used is a reversed-phase column? Maybe, but only for *neutral* molecules. For ionized species I would expect ion-exchange or ion-exclusion behavior to predominate.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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- Posts: 85
- Joined: Wed Oct 19, 2011 9:46 am
Totally agree. Thank you.L21 is polystyrene (OK, PS-DVB). The PRP1 is clearly what is indicated for that classification. Hamilton claims the PRP X100 as also being L21, but I would argue that is inappropriate (the intent of L21 is a neutral resin).
Could the X100 be used is a reversed-phase column? Maybe, but only for *neutral* molecules. For ionized species I would expect ion-exchange or ion-exclusion behavior to predominate.
Q. F. Ignacio Viera
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