Hamilton prp columns
Posted: Wed Oct 19, 2011 2:14 pm
by qfiviera
Does anyone know the difference between hamilton prp x100 and prp-1? Because I need a L21 column for Alendronate USP assay and I have this two in my laboratory? Which one is better?
Re: Hamilton prp columns
Posted: Wed Oct 19, 2011 9:44 pm
by tom jupille
The information is available on the Hamilton web site:
prp x100:
http://www.hamiltoncompany.com/products ... ies/c/369/
prp1:
http://www.hamiltoncompany.com/products ... ies/c/274/
The short answer is that PRP X100 is an anion exchange column; PRP1 is a reversed-phase column (entirely different animals!)
Re: Hamilton prp columns
Posted: Thu Oct 20, 2011 8:52 am
by qfiviera
Thanks for your answer Tom. But, PRP X100 can still be used in reversed phase, isn´t it?
Re: Hamilton prp columns
Posted: Thu Oct 20, 2011 10:24 am
by tom jupille
L21 is polystyrene (OK, PS-DVB). The PRP1 is clearly what is indicated for that classification. Hamilton claims the PRP X100 as also being L21, but I would argue that is inappropriate (the intent of L21 is a neutral resin).
Could the X100 be used is a reversed-phase column? Maybe, but only for *neutral* molecules. For ionized species I would expect ion-exchange or ion-exclusion behavior to predominate.
Re: Hamilton prp columns
Posted: Thu Oct 20, 2011 2:23 pm
by qfiviera
L21 is polystyrene (OK, PS-DVB). The PRP1 is clearly what is indicated for that classification. Hamilton claims the PRP X100 as also being L21, but I would argue that is inappropriate (the intent of L21 is a neutral resin).
Could the X100 be used is a reversed-phase column? Maybe, but only for *neutral* molecules. For ionized species I would expect ion-exchange or ion-exclusion behavior to predominate.
Totally agree. Thank you.