chemstation does not pick up peaks automatically

[nora87]

hello there:i am having a problem with my chemstation, it doesn't pick up peaks automatically, i need to manually integrate all the peaks manually, even the ISTD peak which is not good, so i was wondering how i can change the settings in my chemstation so it can pick up peaks, or is that a method issue?
i really appreciate your help

ps: msd chemstation version that im using is MSD chemstation G1701DA revision D.01.00

thanks!!!

[Johnny Rod]

Have you tried altering the Threshold setting in the integration settings? Also Peak Width?

[lmh]

which chemstation are you using, LC or GC?

[dblux_]

which chemstation are you using, LC or GC?

As far as I know MSD chemstation G1701DA is only for GC.

As Johnny Rod advised earlier play with integration parameters (Chromatogram>MS signal integration parameters ...).

Try to set lower values for Initial threshold and for area and hight rejections).

Then integrate peaks from menu (Chromatogram>Integrate).

Good luck

[nora87]

thank you so much guys for the help, it worked . I have another question, now this is a different GC, when i scan in my batch first, and click ok it saves my initial sequance which is fine, but the problem is when i go again to the sequance table and add more samples and hit ok, it comes up with error msg mstopcmd.dll: vial is out of range, altho my sequance is in order, and i have to restart my program every single time when i want to scan in some sample, because i only get one time save each time and thats very time consuming. do you guys have any idea about this error massage? im thinking its a software issue or could be macros... i would really appreciate your guys' help

ps. the GC msd chemstation is G1701EA E.02.00.493

Thanks!!!!

[lmh]

doh, sorry, I should read properly before asking for further details that the original poster had already included...

[Steve Reimer]

The EA version has changed how it looks at the vial column. It used to ignore it unless the autosampler was used. In my case I'm running VOAs so Chemstation doesn't control my P&T. In the DA version I used vial #s to track the sequence order for my report forms. With the upgrade to EA my vial # must be 1 for every line in the sequence. Of course when I use the repeat line and increment command Chemstation increments the vial then gives an error if I don't change it before saving. And no, you can't eliminate the vial column, it doesn't like that either. The error is "vial is out of range".
imho that's a software bug that should be fixed.

[MX304]

thank you so much guys for the help, it worked . I have another question, now this is a different GC, when i scan in my batch first, and click ok it saves my initial sequance which is fine, but the problem is when i go again to the sequance table and add more samples and hit ok, it comes up with error msg mstopcmd.dll: vial is out of range, altho my sequance is in order, and i have to restart my program every single time when i want to scan in some sample, because i only get one time save each time and thats very time consuming. do you guys have any idea about this error massage? im thinking its a software issue or could be macros... i would really appreciate your guys' help

ps. the GC msd chemstation is G1701EA E.02.00.493

Thanks!!!!

Are you using the sequence table that looks like an excel sheet, or the older original Chemstation sequence table?

[nora87]

thanks alot for ur guys' help i really appreciate it. Answering your question mx304, i am using the chemstation sequence table, and I also checked the other GC-MS's that we have, and there is one that have the same version of chemstation and it works fine, i really don't know why im having the problem with mine.

[MX304]

thanks alot for ur guys' help i really appreciate it. Answering your question mx304, i am using the chemstation sequence table, and I also checked the other GC-MS's that we have, and there is one that have the same version of chemstation and it works fine, i really don't know why im having the problem with mine.

If it works fine on other instruments you may have a corrupt system file somewhere. One easy thing to try is to open the configuration editor, click through all the options without making any changes, then let it update. This will replace any bad Chemstation files without messing up or changing any of your settings. You will need to tune the instrument again after closing the config editor since it will reset the tune file to default settings.

[Yama001]

I believe the newer version of the chemstation will place a backup of your tunes so you may retrieve them after configuration. For example, look in MSDChem\1\5973.OLD. Much better than the older systems that would just wipe it out

OTOH, I never trust software, so I just grab whatever tune file I am normally using and make a copy elsewhere.

Chemstation does have an autointegrate feature as well - it is by no means perfect, but it can be useful to get your integration settings into a ball park range. Note also that if you use the RTE integrator, the default settings use an area reject based on percent of the largest peak, so if you have a large solvent peak, you tend to miss everything else.

Tim

[MX304]

I believe the newer version of the chemstation will place a backup of your tunes so you may retrieve them after configuration. For example, look in MSDChem\1\5973.OLD. Much better than the older systems that would just wipe it out

OTOH, I never trust software, so I just grab whatever tune file I am normally using and make a copy elsewhere.

Chemstation does have an autointegrate feature as well - it is by no means perfect, but it can be useful to get your integration settings into a ball park range. Note also that if you use the RTE integrator, the default settings use an area reject based on percent of the largest peak, so if you have a large solvent peak, you tend to miss everything else.

Tim

You are correct that it will save the tunes, but if I had a dollar for every time the backup didn't work..... I always re-tune just to be sure.