Effect of TFA concentration on RP separations of peptides
Posted: Tue Oct 11, 2011 3:33 pm
by leonvdbos
How critical is the effect of the TFA concentration on the retention time and separation of peptides on a reversed phase column? Will you see a difference when the amount of TFA is 0.1% instead of 0.2%? And if so will you also see a difference when the amount of TFA is 0.12% instead of 0.1%?
Re: Effect of TFA concentration on RP separations of peptide
Posted: Tue Oct 11, 2011 3:40 pm
by tom jupille
How much (if any) difference you will see depends on your peptides. Among other things, TFA acts as a weak ion-pairing reagent, so increasing the TFA concentration will increase retention of more basic peptides.
Re: Effect of TFA concentration on RP separations of peptide
Posted: Tue Oct 11, 2011 6:46 pm
by DJ
Re: Effect of TFA concentration on RP separations of peptide
Posted: Wed Oct 26, 2011 3:01 pm
by leonvdbos
Thanks! the handbook is very useful! Sorry for the late respons..