wavelenght accuracy determination.

[mirac_han]

ı have a agilent 1200 hplc system. ı red some topics about wavelengt accuracy. ı bought a cafeıne standart. ıt has a certificate standart. ı wıll look a spectrum my standart. my hplc detector is uv variable wavelenght. can ı see the max and min absorbance unıt my detector? . my detector isn't a diodearray detector.
thanks for your answer.

[bisnettrj2]

With the VWD you can insert a scan function into the detector timetable. You can specify the time at which to do this, or you can stop the flow of the pump when the peak is in the cell and perform the scan. You can specify the range across which to scan (probably 190-400nm for the full range that the VWD can cover). You can view the spectra at the time the peak is in the flow cell using the Online Spectra window. However, I believe you need Chemstation with the Spectral Tools module installed to view the acquired UV spectra offline. See page 85 of the pdf in the link below:

http://www.chem.agilent.com/Library/use ... _ebook.pdf

Also from the pdf - "Wavelength accuracy - ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter". If you are able to run this VWD scan and your UV-max doesn't match what is described on the certificate you have (within ± 1 nm, that is), you might want to run the VWD calibration under the Diagnostics section of the Chemstation software.

[carls]

I'm fairly sure the Chemstation software has a built-in function to check the wavelength accuracy using a holmium oxide filter present in the detector. Depending on the version of Chemstation you're using it will either be in the "diagnosis(?)" section of chemstation or in the Agilent tools/lab advisor software. This is likely also available using the handheld controller if you have one.

[bisnettrj2]

Also from the pdf - "Wavelength accuracy - ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter". If you are able to run this VWD scan and your UV-max doesn't match what is described on the certificate you have (within ± 1 nm, that is), you might want to run the VWD calibration under the Diagnostics section of the Chemstation software.

Got it covered, carls, but it is a good point to re-iterate - if you don't calibrate your VWD per Agilent's specs before the test sample, the results mean nothing (according to Agilent, that is...). And if you calibrate after the test sample, you still need to run the test sample again to confirm the calibration. Although, performing the calibration technically only involves the deuterium lines and holmium oxide filter, according to Agilent. The caffeine wavelength test might be a nice external test, but I don't think it's one you would be able to hold Agilent to, unless you got an engineer to repeat it on-site with your instrument. Are you required to run this test for some regulatory agency requirement, or is this only for your own personal satisfaction?