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Fractionation with silica..whats this called SEC??
Posted: Wed Oct 05, 2011 11:05 am
by seamoro
Hey im typing up my cv and im just wondering what i call "Fractionation of mushroom sample using column with silica and using increasing polarity of solvents to elute fractions"...what is this called....is this size exclusion chromtagraphy (SEC)? or column fractionation?
Re: Fractionation with silica..whats this called SEC??
Posted: Wed Oct 05, 2011 12:53 pm
by tlahren
It is typically called solid phase extraction/fractionation (SPE). The separation with silica gel is based on polarity not molecule size. This is why you fractionate with different polarity solvents. I believe a molecular sieve is used as a stationary phase for Size Exclusion Chromatography but I'm not too familiar with it. Good luck with the job hunt.
Ty
Re: Fractionation with silica..whats this called SEC??
Posted: Wed Oct 05, 2011 4:37 pm
by KDF
This is normal phase chromatography. If used at moderate to low pressure as a clean up procedure, it is typically called Flash Chromatography.