SPME Questions

[Ruth1770]

I am considering using SPME as a way of trapping compounds coming off my samples as they are heated and I have a couple of questions...

1. Could I leave my SPME fibre in the headspace above the sample or immersed in a chopped up solid sample for a number of weeks at 80C or will this cause the degradation of the fibre?
2. I might want to be able to subject my resulting fibre to a number of techniques. Can the fibres be extracted in a range of solvents?


Many thanks.

[Alexandre]

1 in the gas phase but need to mix, at least occasionally
2 yes, treat this as a sort of SPE cartridge

[Ruth1770]

Thanks for the reply. If I do extract the fibre in solvent, can the fibre be reused? Would I need to heat it at a high temperature under vacuum to recondition it?

[bryan12345]

some solvent might swell the fiber.

[AICMM]

Ruth1770,

So, are you saying that your chopped up whatever won't reach equilibrium for the component you are interested in for several weeks?

Best regards,

AICMM