Fractionation with silica..whats this called SEC??

[seamoro]

Hey im typing up my cv and im just wondering what i call "Fractionation of mushroom sample using column with silica and using increasing polarity of solvents to elute fractions"...what is this called....is this size exclusion chromtagraphy (SEC)? or column fractionation?

[tlahren]

It is typically called solid phase extraction/fractionation (SPE). The separation with silica gel is based on polarity not molecule size. This is why you fractionate with different polarity solvents. I believe a molecular sieve is used as a stationary phase for Size Exclusion Chromatography but I'm not too familiar with it. Good luck with the job hunt.

Ty

[KDF]

This is normal phase chromatography. If used at moderate to low pressure as a clean up procedure, it is typically called Flash Chromatography.