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Column Bleeding

http://www.chromforum.org/viewtopic.php?t=17820

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Column Bleeding

Posted: Fri Sep 30, 2011 6:00 am
by Davidantony
Dear All,
In one our C-18 column(Agilent zorabax SB C-18 2.1x150mm,5ยต)it is found that the column particles are coming outside.The maximum flow is 0.4ml/min.Why this type of problem coming how to stop this type of column bleeding

David Antony

Re: Column Bleeding

Posted: Fri Sep 30, 2011 12:20 pm
by Bigbear
I hate to ask, but have you installed it correctly? Some manufacturers put a frit with larger pore size in the inlet . If that is your case some packing could leak from the column.
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