Chromatography Forum

dear all,
iam trying do bucal release studies in artificial saliva.iam preparing the artificial saliva with xantan gum,potassium chloride,calcium chloride,Dipottasiium ortho phosphate ph is 7.30.

when iam injecting the sample directly iam getting back pressure in new clumn.can any body suggest me what to do

Xantan gum in not something that you want to inject on a LC-column. You need to remove that from the sample before you inject. I would try either some kind of filtration or enzymatic digestion of the xantan gum.

I can rememeber that we solved a similar issue with soluble starch once, by adding amylase. In that case the amylase peak did not interfer with the analysis, and the solution became viscous like water after treatment. I have no idea what enzyme that would digest xantan gum though.

thank you very much i will try some procedure to extract the xantan gum before injecting.