Chromatography Forum

Hi, we are planning to purchase new HPLC can anyone tell me which HPLC is the best.

Thank you.

I just want to know: what is the most common, most purchased, or most popular LC system in the world? Have anyone or institution ever collect the data about this? thanks in advance.

We just got new Waters Alliance HPLCs in December last year, so far they're great.

Hi, we are planning to purchase new HPLC can anyone tell me which HPLC is the best.

Thank you.

The "best" HPLC is the one that best suits your needs.

What are your requirements on the HPLC and what are the samples you are dealing with?

What is the pressure range you need to work with? How about the flow rate range? Do you prefer low-pressure mixing or higher pressure mixing? Loop-based or flow-through-needle type injectors? Which detectors do you need? UV/PDA? fluorescence or ELSD?

If you are mainly doing "bio" separations you will need the "bio" versions of HPLC, the ones with a non-SS fluid path.

That said, the Waters Alliance 2695 and Agilent 1200 (Not the 1260 series) are the most commonly used.

Bear in mind that it is not only the HPLC instrument that you are choosing. The software that comes with your instrument can have huge impact on your productivity.

Cheers,

Hi,
if you r going to use the system for R&D purpose go for agilent.if it is for QC purpose choose Waters.Orelse Shimadzu for Preparative.

if you r going to use the system for R&D purpose go for agilent.if it is for QC purpose choose Waters.

could you give the reason? thanks.

I have worked with Waters, Agilents and Dionex systems. I much prefer the Agilent however I wouldnt pick the Agilent software, I find the Chromeleon far more user friendly as do my team as we have both in my lab at the moment. The Agilent systems are pretty robust and parts are fairly easily attainable. We use an 1100 and 1200 for our standard QC analysis.
That being said I have worked with waters as well and its pretty much all automated and they're pretty good systems too, however I find them harder to "get into" to fix things, but can prise apart the Agilents easier to get at things.
Personal preference I suppose it just depends what you prefer they're all pretty good systems!

lynz x

We just got new Waters Alliance HPLCs in December last year, so far they're great.

I would be interested why you made this decision. I do not understand why people are still buying expensive technology from the 80's. Current entry-level systems will provide you with comparable or better performance for half the price.