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Solid phase extraction (SPE)

http://www.chromforum.org/viewtopic.php?t=17653

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Solid phase extraction (SPE)

Posted: Sun Sep 11, 2011 8:57 am
by Sarah1233
Hi all

Iam trying to seperate a weak acid from plasma. Does any has experience with SPE, I tried to use sep pak but it doesn't give me the required sensitivity, any suggestions.

Susan

Re: Solid phase extraction (SPE)

Posted: Sun Sep 11, 2011 1:02 pm
by Vlad Orlovsky
what is pKa of your acid? You can inject plasma directly into Primesep D mixed-mode column. Plasma proteins will repel from the stationary phase and elute close to the void and acid will retain by anion-exchange mechanism. Something like this:
http://www.sielc.com/Technology_DirectP ... lysis.html
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