Removal Of Trace Oxygen From HPLC Mobile Phase

[Russell1234]

We are needing a HPLC %area purity method on a VERY oxygen sensitive compound. We have premixed and sonic de-gassed our phases (MeCN & Water), and are using an in-line vacuum degasser. However, we are still seeing significant oxidation of our product. Does anyone have any experience of removing even more oxygen from the mobile phase?

[usuchrom]

Hi,
You can probe using a vacuum system as same time you are sonicating the mobile phase. It is very effective.

Regards

[DR]

Or just displace the oxygen w/ helium (continuous low flow He sparge after initial degassing).