Different injection volumes for Calibration curve and sample

[Juan2011]

Dear all,
I am developing a method for estimation of free monomer from a polymer sample. The samples have very wide range of polymers from 10% to 60% of the total content of the sample. Others are recipients. The injection volume of my method is 10µ. The range of is 1 ppm (LOQ) to 25ppm. The sample which has only 10% of polymer does not fit into the curve as it is below 1ppm. I cannot increase the sample amount because it has problem during the extraction process. I tried to inject 20µl of the sample and now it fits into the range of my method. Now I am thinking to go ahead with the validation. Is this ok or anybody experience something like this? I have never done it before, so please just give all your comments and thoughts.
Thank you all.

[tom jupille]

Why not just inject 20 microliters for both samples *and* standards?

[Juan2011]

Hi Tom,
Thank you very much for the reply. I can not inject same voluyme of both the standard and sample because the sample will still be out of range , I mean it is below the CC-1(LOQ). beacuse I am just shifting the intercept and not the slope. ((((((((((((((((((((

[AA]

Dilute your standards and inject the same for both.