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Removal Of Trace Oxygen From HPLC Mobile Phase

http://www.chromforum.org/viewtopic.php?t=17588

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Removal Of Trace Oxygen From HPLC Mobile Phase

Posted: Wed Aug 31, 2011 6:23 am
by Russell1234
We are needing a HPLC %area purity method on a VERY oxygen sensitive compound. We have premixed and sonic de-gassed our phases (MeCN & Water), and are using an in-line vacuum degasser. However, we are still seeing significant oxidation of our product. Does anyone have any experience of removing even more oxygen from the mobile phase?

Re: Removal Of Trace Oxygen From HPLC Mobile Phase

Posted: Wed Aug 31, 2011 8:40 am
by usuchrom
Hi,
You can probe using a vacuum system as same time you are sonicating the mobile phase. It is very effective.

Regards

Re: Removal Of Trace Oxygen From HPLC Mobile Phase

Posted: Wed Aug 31, 2011 3:34 pm
by DR
Or just displace the oxygen w/ helium (continuous low flow He sparge after initial degassing).
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