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which compressibility use with buffer and NaCl 0.15 M

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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Hi I have a little question. I use a phosphate buffer with NaCl 0.15 M in HPLC-SEC of a protein with 0.4 ml/min of flow rate. My hplc pump is a 2250 Bischoff and has a correction for compressibility, still now I used a compressibility as it was water but I think I am wrong. How can I know the correct number ? Do I suppose to use a less value than water ?
Thanks for your anserws.
QF Ricardo Alcalde
At that level of salt, the compressibility of water remains unchanged.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
2 posts Page 1 of 1

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