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which compressibility use with buffer and NaCl 0.15 M

http://www.chromforum.org/viewtopic.php?t=17558

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which compressibility use with buffer and NaCl 0.15 M

Posted: Sat Aug 27, 2011 6:39 pm
by RAlcalde
Hi I have a little question. I use a phosphate buffer with NaCl 0.15 M in HPLC-SEC of a protein with 0.4 ml/min of flow rate. My hplc pump is a 2250 Bischoff and has a correction for compressibility, still now I used a compressibility as it was water but I think I am wrong. How can I know the correct number ? Do I suppose to use a less value than water ?
Thanks for your anserws.
QF Ricardo Alcalde

Re: which compressibility use with buffer and NaCl 0.15 M

Posted: Wed Aug 31, 2011 7:03 am
by tom jupille
At that level of salt, the compressibility of water remains unchanged.
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