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Cleaning validation method by HPLC
Posted: Mon Aug 08, 2011 1:42 pm
by Murugan Saravanan
Hello friends,
Can anyone have experience in the detection level of HPLC method developed for cleaning validation? I appreciate your thoughts in advance.
Warm Regards
Murugan Saravanan
Re: Cleaning validation method by HPLC
Posted: Mon Aug 08, 2011 7:15 pm
by JGK
Are you attempting to prove that an equipment item has been cleaned effectively?
If so you need to validate your sample extraction at your detection methods LOQ. This will ensure that cleaning swabs will give you an accurate measure of equipment contamination.
Re: Cleaning validation method by HPLC
Posted: Tue Aug 09, 2011 7:40 am
by Murugan Saravanan
Thanks, JGK
Warm Regards
Murugan Saravanan
Re: Cleaning validation method by HPLC
Posted: Tue Aug 09, 2011 12:37 pm
by Antje_Traub
We developed and validated a lot of hplc methods for analysis of cleaning solutions and swab extract solutions. In most cases we have a LOQ of 0,1 (rinse)- 1 ppm (swabs).