Cleaning validation method by HPLC

[Murugan Saravanan]

Hello friends,
Can anyone have experience in the detection level of HPLC method developed for cleaning validation? I appreciate your thoughts in advance.
Warm Regards
Murugan Saravanan

[JGK]

Are you attempting to prove that an equipment item has been cleaned effectively?

If so you need to validate your sample extraction at your detection methods LOQ. This will ensure that cleaning swabs will give you an accurate measure of equipment contamination.

[Murugan Saravanan]

Thanks, JGK

Warm Regards
Murugan Saravanan

[Antje_Traub]

We developed and validated a lot of hplc methods for analysis of cleaning solutions and swab extract solutions. In most cases we have a LOQ of 0,1 (rinse)- 1 ppm (swabs).