Effect of flow rate on peak area
Posted: Tue Aug 02, 2011 6:55 pm
I understand that the peak area decreases with increasing flow rate using a UV detector for example. Which flow rate should i use for a calibration curve?
The same rate you plan to use for your samples.
If you want to learn more, investigate Van Deemter plots for the size (length, width, particle size) LC column you are playing with.