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- Posts: 51
- Joined: Mon Dec 07, 2009 12:46 pm
it likes MeCN, but once I add aqueous it gets annoyed
should I think about doing this normal phase?
any thoughts/suggestions welcome
C
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Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
For my applications, I have made different observations. When the sample is not solved in the mobile phase, you can try much more when you work in reversed phase HPLC. In RP-HPLC you have more possibilities for the sample solvent. If the OP will post more Information, it will be easy to tell him a more proper advice. And sureley you can have advantages to use RP-HPLC, also when your sample is solved in e.g. tolueneAnd if that doesn't work, yes, solubility issues are a valid reason for using normal phase.
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