Chromatography Forum

Hi everybody,

I have a problem with analysing the related substances of cefixime . I have performed the method on EP, however it did'nt worked. According to EP, cefixime has 6 impurity. Altough I have followed all the parameters (column, mobile phase, wavelengt, column temperature, etc.) the same as in the EP but some impurity peaks have been detected on the same RT and I couldn't have seperated them. How can I seperate them, or do anyone have another method for the related substances of cefixime?

Can you help me,please?

Thank you...

Maybe this will help:
PDF]
A Validated RP – HPLC Method for Simultaneous Estimation of ...
- [ Diese Seite übersetzen ]

www.e-journals.in/open/vol5/no3/0752R-648-651.pdf
Dateiformat: PDF/Adobe Acrobat - Schnellansicht
von G RATHINAVEL - Zitiert durch: 5 - Ähnliche Artikel
Abstract: This paper presents a RP-HPLC method for the simultaneous estimation of cefixime and cloxacillin in tablets. The process was carried out ...

Thank you for your attention..

I will also try the method you have offered.

Maybe this will help:
PDF]
A Validated RP – HPLC Method for Simultaneous Estimation of ...
- [ Diese Seite übersetzen ]

http://www.e-journals.in/open/vol5/no3/ ... 48-651.pdf
Dateiformat: PDF/Adobe Acrobat - Schnellansicht
von G RATHINAVEL - Zitiert durch: 5 - Ähnliche Artikel
Abstract: This paper presents a RP-HPLC method for the simultaneous estimation of cefixime and cloxacillin in tablets. The process was carried out ...

Dear pecurie,

EP method of Cefixime RS is same as that of USP RS method which is also same as that of JP.
Pl. check the columns given in USP(superspher, merck, 125 x 4.0mm, 4µ) as well as work with Buffer: Solvent ratio for better separation.
Have a good luck.

Regards
Murugan Saravanan