Chromatography Forum

Hi,

Has anyone tried to quantify vitamin D in blood collected on EDTA tubes, or serum maybe?
Which kit have you tested? Can you give me a company name? And some ideeas about LOD and LOQ? I have an LC-TQ-MS and I have to try it, validate it for clinical determinations-children especially but I need a good kit to start with- a good column

Thank,

Simplified HPLC method for quantitation of vitamin D in blood serum.

Lukaszkiewicz J, Bibik K, Lorenc RS.


Source

Monument Hospital Child's Health Centre, Warszawa-Miedzylesie, Poland.


Abstract

A single-step high performance liquid chromatography (HPLC) method for vitamin D quantitation in blood serum is described. Methanol/hexane extraction, silica Sep-Pak purification, differential dissolution, filtration, and the use of a precolumn in place of an injector sample loop allow for using only one (reversed phase) HPLC step instead of the usual two. Detection limit with the HPLC system used was about 1 micrograms/L of serum. Recovery of vitamin D3 added to serum ranged from 90% to 109%. Intra and interassay coefficient of variation values were 8.1% and 15.4%, respectively.


PMID: 2547118 [PubMed - indexed for MEDLINE]
Hope that will help

thank you