SPME fibre

[Palash@massey]

Hello everyone,

I am a new user of SPME fibre - analysing tomato volatiles. I bought 3 SPME fibre from Supelco last week. Last night, after 6-7 injection, I forgot to retract SPME fibre from injection port – it was sitting on the injection port overnight (am doing manual injection). When I came back today morning, I noticed fibre inside got burned out and blackened. What could be the reason of that? I am using oven temperature 260°C as final temperature of my sample run.
Do you think I can use that fibre or I need to throw that out?

best regards
Pal

[erica]

Hi Paul,

What is the phase of this fiber? It is known that polyacrylate fibers get brownish after use. Do you have a QC check for your method? If you have, you can run your QC and compare it with previous results to see whether the fiber performance has changed or not. I would say you lost your fiber in the case it is too much burnt, but a QC check can confirm you this.

Good luck!!!

Erica

[Don_Hilton]

Note that the fiber was at the inlet temperature. Check the literature on the fiber and see what it has for temperature limits - that may help.

[Peter Apps]

What was the inlet temperature ?, the final oven temperature is not the temperature that the fiber was exposed to.

Peter

[kabdesslam]

in first place you should 've have seen the manufacturer ' recommendation on how to activate the fibre (am sure with seplco there is always a recommendation ) so check them .and try the QC approach as well but I think from my experience that u lost ur fibre