Vo and Vt standards for FPLC

[burroa03]

Other than blue dextran (Vo) and tryptophan (Vt), what other standards can be used for FPLC? Thanks in advance.

[Mike in Seattle]

What type of separation technique are you using? From the standards you have chosen I'm guessing your are doing Gel Permeation or Size Exclusion Chromatography.

Are you trying to establish a molecular weight/stokes radius calibration curve?

Do you have a range of sizes in mind?

[burroa03]

I am using FPLC to look at HDL particle size. These sizes range from <7.1nm to >12.6nm.

[Andy Alpert]

You can find examples of standards in a book chapter I wrote on SEC. Here's the link: http://www.polylc.com/downloads/SEC_boo ... r_ver1.pdf

Andy Alpert