extraction ointment or cream

[che313]

can any body recomend procedure for extraction ointment or cream for gc-ms or lc-ms?

[Consumer Products Guy]

We'd try methanol first. If that didn't dissolve your sample, our next tries would be DMF or THF.

[DSP007]

What is active components ?
What is basic of ointment ? Emulsion (water /oil, oil/water) , lipofilic melt (parafine, lanoline) , hydrofillic true solution in PEG , thickened with Carbomer true aqueous solution ?


Methanol is good solvent from solved in methanole substancies (such as menthol).
Good operation - will cold extract by - 10 to - 30 C.

[Consumer Products Guy]

What is active components ?

Oftentimes a poster cannot detail what his analyte or his sample matrix is, because it could tip his hand to a competitor. For example, if a company was going to come out with a new product, like a new "category" and thus have a head start on the competition, he would need to keep that information as proprietary, to get a business head start.

Let's say Jergens Lotion wanted to come out with a sunscreen product (and maybe they already have one) - Jergens wouldn't want the competition to know in advance.

[DSP007]

Yes of course. But
1) Never ask full recept. But answer : "it painless drug ( aminobenzoic acid derivates in hydrochloric salt form) and kumarines in lipid base" - full complite answer from useful dialog
2) If it is "big commercial secret" - no problem. You will have good money from patents - and you job problem => only you commercial problem

[GOM]

Hi,

Try methanol/dichloromethane (70/30) or 50/50. It often works for me

Cheers

Ralph

[Peter Apps]

can any body recomend procedure for extraction ointment or cream for gc-ms or lc-ms?

Do you want to extract it or dissolve it. If the first then you need a solvent that dissolves the analyte, but not the matrix, if the second than a solvent that dissolves both. If you just dissolve it you may need to do a clean up to get rid of components that might crud up your instruments.

Peter

[GOM]

Hi,

Just to expand on my previous reply.

As Peter correctly pointed out it depends on your target analyte. I used the methanol/dichloromethane mix based on on Restek's "Magic mix" because it can dissolve aqueous/organic samples. Since creams are usually oil in water or water in oil emulsions often based on, but not limited to, (wow I sound like a patent) low melting point paraffin, cetyl/stearyl alcohol, wax esters, dimethicone or other silicones it worked very well to dissolve the sample. Depending on the target analyte and it's solubility I would then play around ( pre- GLP ) by adding more water or methanol. This would then form 2 phases with the analyte hopefully in one of the phases with the least extra crud.

I must admit that in later years with GCMS/SIM I did become a bit lazy about what I put on the column

If you could tell us what your target analyte is then CPG might be able to help you more easily

Regards

Ralph

[cooky_ju_juve]

Hi, please help me.

I have a problem with how to separate active ingredient(prefer soluble in methanol) from mixture of orange oil and non-ionic surfactants. Since the orange oil make baseline noise at the same time as retention time of active ingredient, which interrupt peak area calculation. Thus, I want to reduce the interfere by separating oil from sample, but I don't know how to. Please help me!

Thank you

Nattawee

[Consumer Products Guy]

I have a problem with how to separate active ingredient(prefer soluble in methanol) from mixture of orange oil and non-ionic surfactants. Since the orange oil make baseline noise at the same time as retention time of active ingredient, which interrupt peak area calculation. Thus, I want to reduce the interfere by separating oil from sample, but I don't know how to.

If you really need help, detail
(1) what is your active ingredient
(2) are you using GC or HPLC, and/or do you have access to both? If HPLC, what detectors does your company have?

[cooky_ju_juve]

Hi

My active ingredient is ellagic acid, and I'm using HPLC with uv-detector.

Thankyou for your kindness

nattawee