I ama young scientist and a new Totalchrom user, and I am struggling with how to go about creating a custom expression to automatically calculate wt./wt.% assay. I understand how to add custom expressions in a report format. As of right now I am setting my reference standard calibration level to "1", and added in the amount of standard used. In my sequence my first reference standard is set to "cal-replace" and the next four are set to "cal-average". I have added sample amounts into the sequence, and understand that the method will have to be calibrated to the working standards each time it has run.
In the past, I was told to use the following expression:
(#RA/#WT) x 100
It was explained to me that raw amount, in totalchrom terms:
area sample/wt.standard x area standard
Unfortunately, my weight percent values do not appear to be correct. Has anyone else had success calculation weight percents using totalchrom?
Any help is sincerely appreciated.